Determination of Methamidopho in Water by Micellar Electrokinetic Capillary Chromatography / 环境与健康杂志

ABSTRACT

Objective To develop a capillary electrophoresis method for the determination of methamidopho residue in water.Methods Methamidopho in water was determined by micellar electrokinetic capillary chromatograph.The qualitative analysis was done by retention time and quantitative analysis by external standard curve.Results The optimum conditions for methamidopho determination were pH= 7.5,20 mmol/L phosphate buffer,30 mmol/L SDS,wavelength of 254 nm,separate voltage 25 kV,inject duration of 9 s.The linear range was 1.0-200 ?g/ml,the correlation coefficient was 0.999 1,the rates of recovery were 91.5%-105.2%,the relative standard deviations were 1.35%-3.24% for this method.The limit of determination was 0.007 1 ?g/ml(capillary volume was 3 ?l).Conclusion This method was sensitive,accurate and applicable to rapid the determination of methamidopho.