Effect of Toluene Diisocyanate on Cell Multiplication and DNA Damage / 环境与健康杂志

ABSTRACT

Objective To study the effect of toluene diisocyanate (TDI) on cell multiplication and DNA damage. The present paper also aims to provide the proof for the potential biological effect. Methods The cell growth inhibition rate of toluene diisocyanate (0.04,0.08,0.16 ?mol/ml)was determined with MTT method,the DNA damage of TDI(0.01,0.02,0.04,0.08,0.16 ?mol/ml) was detected by the single cell gel electrophoresis technique (SCGE). Results Toluene diisocyanate caused morphological change of the cells. It could inhibit CHL cells growth with a dose-time-reaction relationship. The cell growth inhibition rate in 0.16 ?mol/ml group was 88.6%. Toluene diisocyanate could induce DNA breakage. The rate of comet tail,DNA content of comet tail and comet tail length in experiment group were higher and showed a dose-response relationship compared with the negative group. The rate of comet tail,DNA content of comet tail and comet tail length in 0.16 ?mol/ml group were 86.30%,34.54% and 7.18 ?m. Conclusion TDI can inhibit the growth of the cells and induce DNA damage.