Study on Correlation between Expression of CD68 and MRI during the Course of Induced Hepatocellular Carcinoma by Diethylnitrosamine in SD Rats / 实用放射学杂志

ABSTRACT

Objective To study the pathological foundations of enhanced MRI with superaramagnetic iron oxide(SPIO) and its diagnostic value in liver diseases.Methods 52 SD rats were divided into 2 groups in random,group A(n=40) bred with 2 ‰ diethylnitrosamine(DEN) to induce hepatocellular carcinoma(HCC),group B(n=12) as control group bred with water.6 rats and 2 rats in group A and group B were killed 6,8,11,14,17,20 week later,respectively,before that every SD rats underwent conventional plain and SPIO enhanced MRI examinations.All specimens were examined by HE staining and immunohistochemistry to test CD 68 with SP.Results The successful ratio of induced HCC was 90% and the mortality was 15%.The rats in group A could be divided into 4 stages,including hepatic fibrosis(HF),liver cirrhosis(LC),hyperplastic nodules and hepatocellular carcinoma(HCC).CD 68 positive cell in the normal control tissue(22.83?4.61/HP) was significantly more than that in LC(11.67?4.64/HP)(P