Micromechanism and biocompatibility of cartilage derived microcarrier for injectable tissue engineering cartilage / 中国矫形外科杂志

ABSTRACT

[Objective]To improve the preparative method of catilage derived microcarrier and evaluate the micromechanism and biocompatibility of this metarial for injectable tissue engineering cartilage.[Methods]Fresh porcine articular cartilage were obtained and shattered in the iso-osmia liquid in 4℃.After gradient centrifugation,150-300 ?m size cartilage micelles were gathered and were subjected to 1% Triton X-100 once and physiological saline twicely.The structure of specimens were observed and assessed by inverted phase contrast microscopy,environmental scanning electron microscope.And the composition of these specimens were stained with haematoxylin-eosin,safranin-O,toluidine blue and immunohistochemistry of collagen type Ⅱ.The microcarriers were seeded with rabbit bone mesenchymal stem cells(BMSCs) and cocultured with Rotary Cell Culture System(Rotary Cell Culture System,RCCS TM).[Results]The cartilage particles had fiocculus apparence.There were numerous villus on the surface of these cartilage micelles,which were stained positive with the immunohistochemistry of collagen type Ⅱ.The inner part of these cartilage micelles were stained positively with safranin-O and toluidine blue.After coated with BMSCs and cultured in the RCCSTM,the cells grew well on the surface of the cartilage micelles and the latter could be disperse again after blowed with pipette.[Conclusion]The cartilage micelles,which have large surface area and good boicompatibility,is a new kind of microcarrier for injectable tissue engineering cartilage.