The effects of nicotine on the proliferation and fibronectin synthesis of human periodontal ligament cells cultured in vitro / 实用口腔医学杂志

ABSTRACT

Objective:To observe the effects of nicotine on the proliferation and fibronectin(Fn) synthesis of human periodontal ligament cells(PDLCs) cultured in vitro,and to investigate the mechanism of the effects of smoking on the periodontitis.Methods:PDLCs were cultured in the presence of nicotine at various concentrations,the proliferation of cells was measured by MTT chromatometry,and the synthesis of Fn was measured by ELISA and reverse transcription-polymerase chain reaction(RT-PCR).Results:①MTT chromatometryNicotine inhibited the proliferation of PDLCs obviously(P