Combined Lethal Toxicity of TNF and LPS in Tumor-Bearing Mice / 第二军医大学学报

ABSTRACT

Both TNF and LPS are toxic to host, especially to tumor - bearing host Intravenous administration at a dose of 5?g of TNF or 25?g of LPS could not result in death of mice transplanted with sarcoma 180 (S180) 10 d later, but 80% of S180-bearing mice died after iv injection of 5?g of LPS combined with 0.5?g of TNF. In contrast to TNF, combined toxicity was seriously LPS-dose dependent Combined mortality dropped from 80% to 14% when LPS dose reduced to 1?g, however, 50% of mice died of combined toxicity when only TNF dose dropped to 0.1?g. It suggested that TNF sensitized host response to toxic effect of LPS. Indomethacin could partially block the combined toxicity, demonstrating that combined toxicity of LPS and TNF was partially mediated by prostaglan-dins.