Morphological study on the angiogenesis of peripheral nerve affected by nerve growth factor / 中国矫形外科杂志

ABSTRACT

[Objective]To discuss the effects of angiogenesis about nerve growth factor(NGF) during the peripheral nerve regeneration.[Method]Thirty-six Sprague-Dawley rats with 10mm gap of sciatic nerve were randomly divided into two groups which had been bridged with the new double channel nerve conduit of fusiform shape.Each group contained eighteen animals,in the first group,200 ?l of chitin for medical use was injected into the conduit,in the second group,the two branches of the conduit contained 100 ?l of the chitin and 5 ?l NGF or ciliary neurotrophic factor(CNTF).At four,eight or sixteen week after operation,the angiogenesis of NGF was evaluated with Hematoxylin and Eosin(HE) staining and electron microscope.[Result]There were not significant differences of the regenerative nerve fibres between two channels in the first groups,but in the second group,the regenerative nerve of NGF branch channel was red,crisp and the nerve of CNTF branch channel was yellow and tenacious.HE staining showed that there were much more new vessel in the regernerative nerve tract of NGF the branch channel,and the regernerative nerve fibre was disorder,there were much more fibroblasts and vessels observed under eletron microscope.[Conclusion]NGF can significantly promote the angiogenesis during the peripheral nerve regeneration,the mechanism may be related to fibroblast.