Oxidative Stress Induced by NaAsO_2 in SV-HUC-1 Cell / 环境与健康杂志

ABSTRACT

Objective To study the state of oxidative stress induced by sodium arsenite (NaAsO2) in SV-HUC-1 cell. Methods MTT assay was used to evaluate the viability of the cells. The level of ROS was detected by staining cells with DCFH-DA. The content of GSH and MDA were measured by DTNB and thiobarbituric acid methods. The activity of SOD was measured by xanthine oxidase method. Results Compared with the control group, the viability cells decreased in all the treated groups (P0.05), the activity of SOD in all the treated groups was significantly decreased (P