Determination of omeprazole with a new flow-injection chemiluminescence method / 西安交通大学学报(医学版)

ABSTRACT

Objective To develop a new flow-injection chemiluminescence(FI-CL) method for the determination of omeprazole.Methods Strong chemiluminescence(CL) signal was found generated when omeprazole was injected into the reaction mixture of KMnO4 and luminol.The experimental conditions that affected the CL reaction were optimized by using FI mode.Results Under the optimum experimental conditions(150cm of mixing tubing,0.15mol/L NaOH,7.0?10-5mol/L luminol,2.0?10-5mol/L KMnO4 and 2.8mL/min of flow rate),CL intensity was linearly related to the concentration within the range of 2.0?10-8-1.0?10-5g/mL.The detection limit was 3?10-9g/mL omeprazole,and the relative standard deviations(n=11) were 1.0% and 2.6% for 1.0?10-7g/mL and 1.0?10-6g/mL omeprazole solutions,respectively.Conclusion The proposed method is simple,rapid,sensitive,and suitable for determining omeprazole content in pharmaceutical preparations.