A simple and easy method for cell apoptosis analysis / 西安交通大学学报(医学版)

ABSTRACT

Objective To introduce a simple and easy method for cell apoptosis analysis as an example with artemisinin to induce apoptosis of human U251 cells. Methods Human U251 cells,cultured in suspension and treated with artemisinin,were freeze-thawed. 30 ?L of 3 mmol/L PI was then added and mixed. After being placed under room temperature for 5 min,the cellular ploidy was analyzed by flow cytometry,and the ratio of cell apoptosis was calculated. Results The ratio of human U251 cell apoptosis induced by artemisinin at a dose of 1?10-4,1?10-5 and 1?10-6mol/L was 33.2%,24.0% and 8.0%,respectively. Conclusion The results showed that artemisinin significantly induced the apoptosis of human U251 cells in a dose-dependent manner. Using this method to analyze cell apoptosis can reduce the procedure of cell treatment and avoid many influential factors. Therefore,it can be regarded as a simple,accurate and rapid method for detecting cell apoptosis.