DETERMINATION OF VITAMIN D2 IN BABYFOOD (FORTIFIED FOOD) BY TWO STEPS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY / 营养学报
Acta Nutrimenta Sinica
;
(6)1956.
Artículo
en Chino
| WPRIM
| ID: wpr-549843
ABSTRACT
An improved method for the determination of Vitamin D2 in baby food (fortified dried milk) was established by using two steps of high-performance liquid chromatography (HPLC). The sample was directly saponified and extracted with benzene. The extracted unsaponifiable matter was firstly subjected to preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) with acetonitrile-methanol (32) as a mobile phase. This first step was for the purpose of clean-up, and a fraction containing Vitamin D2 was collected. The separate fraction was subsequently subjected to analytic HPLC with a Zorbax SIL column (straight-phase type) with isopropanol-hexane (0.8 99.2) as a mobile phase. The Vitamin D2 was separated from other contaminants and determined with ultraviolet spectrograph by the peak height estimated. The recovery rate was 84%, standard deviation 12.57 and coefficient of variation 4.57%. The proposed method was rapid, accurate, and suitable for the determination of baby food.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Acta Nutrimenta Sinica
Año:
1956
Tipo del documento:
Artículo
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