A Comparative Study of the Expressions of ets-2, IGF-Ⅱ, C-myc and N-ras in Human Primary Hepatocelluiar Carcinoma and Tumor-adjacent Tissues / 第二军医大学学报

ABSTRACT

The expressions of ets-2, IGF-Ⅱ, C-myc and N-ras in 12 pairs of human primary hepatocellular carcinoma (PHC) and tumor - adjacent tissues are presented in the present paper. The results showed that there was at least one of the four oncogenes studied overexpressed in the 12 pairs of samples. ets -2 was the most commonly expressed oncogene seen in all the PHC and tumor - adjacent tissues, with 3. 5 and 2.4 kb as the major two bands, which differed from the evenly expressed 4. 5, 3. 5 and 2. 4 kb bands in the normal control livers. In six tumor-adjacent tissues the expression of ets-2 was higher than that in PHC. IGF-Ⅱ was expressed as 5. 0 and 2. 0 kb fetal transcripts in PHC and tumor-adjacent tissues, while in the normal control livers it was 5. 6 kb. One tumor-adjacent tissue had IGF-Ⅱ fetal transcripts expressed but the corresponding PHC had no IGF -Ⅱexpressed, indicating the reverse differentiation in PHC. N-ras was expressed as 4. 0 kb band in 8 out of 12 PHC and in 6 out of 12 tumor-adjacent tissues. In two cases the expression of N-ras was higher in tumor-adjacent tissues than in PHC. 5. 6 and 2. 6 kb N -ras transcripts were also detected in one pair of PHC and tumor -adjacent tissue and in two tumor - adjacent tissues, together with the 4.0 kb transcript. C - myc was expressed as 4. 0 kb band in 9 out of 12 PHC and in 6 out of 12 tumor-adjacent tissues. One tumor-adjacent tissue had higher C-myc expression than PHC. In two PHC 2. 2 kb C-myc transcript was detected besides the 4.0 kb transcript. The roles and relationships of these oncogenes in the carcinoge-nesis of human PHC are discussed in detail.