THE EFFECTS OF ?-ELEMENE ON DNA-BINDING SPECIFICITY AND RNA POLYMERASE INHIBITORY ACTIVITY / 中国药理学通报

ABSTRACT

?-elemene that is an element of the Essential oil of the Cuicu-ma Wenyujin showed appreciable anticancer ability. The experimental results showed that the RNA polymerase activity was inhibited by ?-elemene at concentrations of 0.0074 mol/L~0.0172 mol/L. The study of thermal denaturation indicated that the Tm of DNA was decreased after adding ?-elemene. The fluorescent analysis of ?-elemene bound to DNA showed that the intensity of fluorescence was increased. The peak of absorption of DNA was shifted from 260 nm to 240 nm after treatment with ?-elemene.