Application of Dialysis-type Molecular Species Hybridizable Method in the Genetic Monitoring Studies / 第二军医大学学报

ABSTRACT

By means of the dialysis-type method of molecular species hybridization the individual differ ences of polypeptide chains of globin of SMMC/B (abbreviated as B) mice at the filial generations of F28 and F34, and at the same parental generation Bp (?) and Bp ( + ) as well as F35 (Bf) were investigated. The results of hybridization between mouse Hb and sheep Hb are as follows All the hybrid groups exhibited four zones on polyacrylamide gel electrophoretogram, but all the control groups only two zones. The comparison of electrophoretograms showed that the electrophoretic positions of the new components were the same, indicating ? and ? chains assayed were quite close to each other, and no significant differences among individual and intergenerations were observed in B mice globins. By electrophoretogram analysis of the genetic expression products, we might distinguish preliminarily the genetic variation of ?- and ?-genes.