Excitotoxic Cell Death in Cultured Retinal Neurons
Journal of the Korean Ophthalmological Society
;
: 1987-1999, 1997.
Artículo
en Coreano
| WPRIM
| ID: wpr-55063
ABSTRACT
We examined excitotoxicity, putatively a major mechanism of ischemic neuronal death, in primary rat retinal cultures. Retinal cultures were prepared from newborn rats (day 1 or 2). Exposure of these cultures (DIV8-10)to NMDA or kainate induced neuronal death. Furthermore, MK-801 or CNQX each partially attenuated glutamateinduced neuronal death, suggesting that both NMDA and kainate receptors mediate it. Thy-1(+) retinal ganglion neurons, like neurons as a whole, were equally injured by NMDA and by kainate. However, GABA(+) or calbindin (+) neurons of the inner nuclear layer were resistant to NMDA, but highly vulnerable to kainate. These neurons may have AMPA/kainate receptors that are highly permeable to Ca2+, as they take up cobalt with kainate stimulation. These results suggest that the AMPA/kainate receptor, rater than the NMDA receptor, may mediate this pattern of selective neurnonal death.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Retinaldehído
/
Maleato de Dizocilpina
/
N-Metilaspartato
/
Muerte Celular
/
Cobalto
/
Receptores de Ácido Kaínico
/
6-Ciano 7-nitroquinoxalina 2,3-diona
/
Ganglión
/
Neuronas Retinianas
/
Neuronas GABAérgicas
Límite:
Animales
/
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Año:
1997
Tipo del documento:
Artículo
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