Enhancement and depression of cholinergic transmission by 9-amino-1,2,3,4-tetrahydroacridine in rat superior cervical ganglia / 中国药理学通报

ABSTRACT

Using the conventional intracellu-lar recording techniques in the rat superior cervical ganglion, the effects of 9-amino-1,2,3,4-tetrahydroacridine(THA) on cholinergic transmission were studied. By superfusion of THA 0. 1~1 ?mol ?L-1(n = 26), the f-EPSPs evoked by preganglionic stimulation were facilitated, while 50~100 ?mol-L-1(n= 17) inhibited in a concentration-dependent manner, but 10 ?mol?L-1in 46% cells (n=11) increased and another 54% inhibited. Also exogenous ACh-induced potentials (n=27) were enhanced with THA 0. 1~10 ?mol?L-1, and in 60% (n=10) with 50~100 ?mol?L-1but another 40% depressed. In 7 cells, no enhancement of carbachol-inducedpotentials were found with THA 1~100 ?mol-L-1, while depression in 5 cells by 50~100 ?mol?L-1was observed. Pretreatment of atropine (1?mol?L-1) only partially eliminated THA inhibition on f-EPSPs but not ACh-or carbachol-induced potentials. It was suggested that THA facilitated cholinergic transmission by its anti-cholinesterase action and inhibited by both pre-and postsynaptic mechanism at different concentration levels.