Study on The Effects of Chronic Renal Failure on Hearing and Its Mechanism / 浙江中医药大学学报

ABSTRACT

[Objective]To observe the effects of chronic renal failure (CRF)on hearing and investigate its mechanism.[Methods] Twenty-nine male wistar rats were randomly divided into four groupsⅠthe normal rats as control for 12 days (n=6); Ⅱrats given adenine(ig) for 12 days (n=8); Ⅲthe normal rats as control for 24 days (n=6);Ⅳ rats given adenine(ig) for 24 days(n=9). The rat model of CRF was produced by being perfused of adenine [300mg/kg]for 12d and 24d. Brain-stem auditory evoked potential(BAEP) was utilized to evaluate the auditory function and renal tissue morphology was observed; Serum sampling was used to determine the content of Cr, BuN. The expression of NOSⅡin the cochlear was measured by immunohistochemical technique.[Results] After adenine given for 12d or 24d, the Cr and BuN were in significant higher level than that in control group. The BAEP thresholds were gradually higher than that in control group, the BAEP delitescence prolonged. Light microscopic observation of renal tissue showed that the degree of fibrosis was gradually higher. Data of immune histochemistry about the cochlear tissue showed that the expressions of NOSⅡ were feebly positive in the cochlear vassal texture, inner-outer hairy cell and helical-ganglion cell, and following functional damage of the kidney, their expressions reinforced gradually, amongst which strongest expression was vassal texture, the next helical-ganglion cell, the last inner-outer hairy cell.[Conclusion]CRF can result in deafness. It is likely concerned with toxic effects of the metabolizable substance, the metabolizable dysfunction of the electrolyte, shortage of trace elements, etc. One of the important mechanisms of the effects of CRF on hearing is the toxic damage of NO to cochlea.