Fluorescent measurements of H~+/K~+ exchange in primary cultured inner medullary collecting duct cell monolayers or rabbit kidney / 中国药理学通报
Chinese Pharmacological Bulletin
; (12)1987.
Article
en Zh
| WPRIM
| ID: wpr-551920
Biblioteca responsable:
WPRO
ABSTRACT
AIM To establish the fluorescent measurement technique for H+ /K+ exchange in primary cultured IMCD cell monolayers of rabbit kidney. METHODS To measure H+ /K+ exchange in the primary cultured IMCD cell monolayers under different CO2 concentrations by the BCECF/AM fluorescent probe. RESULTS [pH], recovery rate after acid loaded in the respiratory acidosis group was 0.0620 ? 0.012, and was significantly higher than that of the control group (0.0504 ? 0.009, P 0.05). CONCLUSION The fluorescent measurements of H+ /K+ exchange in primary cultured IMCD cell monolayers of rabbit kidney has many benefits which are convenient manipulation, stable and credit result, economy and safety, this method should further be popularized.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Pharmacological Bulletin
Año:
1987
Tipo del documento:
Article