OBSERVATION OF THE DEVELOPMENT OF PLASMA MIP-1? AND MCP-1 IN RATS WITH PULMONARY FIBROSIS / 解放军医学杂志

ABSTRACT

To investigate the plasma MIP 1?and MCP 1 levels in rats with pulmonary fibrosis produced by bleomycin and preliminarily study the pathogenesis of the two chemokines in pulmonary fibrosis. The rats were randomly divided into 2 groups normal control and pulmonary fibrotic group. Rats were killed on day 1, 3, 7, 14 and 28, and the plasma were collected .The solid phase sandwich enzyme linked immuno sorbent assay (ELISA) was used to assay the level of plasma MIP 1? and MCP 1. The results showed that the level of MIP 1? and MCP 1 in rats with pulmonary fibrosis were significantly higher than those in normal control ; plasma MCP 1 level was significantly correlated with plasma MIP 1?. It indicates that plasma MIP 1? and MCP 1 may be useful markers for monitoring the course of pulmonary fibrosis and they may reflect the clinical evidence of pulmonary fibrosis.