AN ANIMAL EXPERIMENTAL STUDY OF NITRERGIC INNERVATION IN URINARY BLADDER AFTER SPINAL CORD INJURY / 解放军医学杂志

ABSTRACT

This study was undertaken to examine ncNOS IR in bladder after spinal cord injury (SCI). In the meantime, we determine rats bladder ncNOS IR following intravesical instillation of capsaicin after SCI. Adult Sprague Dawley rats and guinea pigs were randomly divided into normal group, sham injury group, spinal cord injury group (4～5weeks after T 8～9 transection). A dose of 1mmol/L intravesical capsaicin was instilled in a part of SCI rats. Bladders of all animals were divided into three tissue pieces bladder base, bladder body and bladder dome,in which the distribution of ncNOS IR was examined. Spinal transection induced a significant ncNOS IR increase in guinea pig bladder base. In rats, the quantity of ncNOS IR did not differ between the two groups. Instillation of intravesical capsaicin can cause significant up regulation of rat bladder ncNOS IR after SCI. The increase of ncNOS IR in SCI animals bladder indicates that NO may play an important role in the regulation of micturition reflex after SCI. In SCI rats, ncNOS IR can significantly be up regulated by intravesical capsaicin instillation, suggesting that NO may act as a factor in the action of capsaicin.