EPITHELIAL CELL PROLIFERATION AND DNA PLOIDY PATTERN IN ESOPHAGEAL TUMOR INDUCED BY GASTRIC REFLUX / 解放军医学杂志

ABSTRACT

The aim is to study proliferation of esophageal epitheliun with reflux of different gastrointestin secretions to the esophagus and its role in carcinogenesis of esophagus. Gastroesophageal reflux(G), duodenoesophageal reflux(D) and duodenogastroesophageal reflux(DG) model and control group(C) were reproduced by operations. All rats were given carcinogen (methyl n amyl nitrosamine). Then their esophagi were harvested at 20, 26,and 40 weeks after the operation. With immunohistochemical staining of SABC method, PCNA labeling indexes(LI) were compared. Epithelial cells were stained with PI and assessed with flow cytometry(FCM).The results of FCM were compared in terms of DNA index(DI), proliferating index(PI) and aneuploidy. LIs increased with time of reflux and the administration of MANA. LIs in reflux groups, especially in D and DG group, were significantly greater than that in the C group with the same time interval. 3 indexes of FCM showed obvious changes in G, D and DG group, and they were greater than C group.The changes ware most marked in D and DG groups.It is concluded that all kinds of reflux into the esophagus can induce abnormal proliferation of esophageal epithelium and enhance carcinogenesis of the esophagus. The role of duodenoesophageal reflux may be more important than gastroesophageal reflux.