THE EXPRESSION OF CYTOKERATIN 18 AND THE RELATIONSHIP BETWEEN THE SERUM LEVELS OF TISSUE POLYPEPTIDE SPECIFIC ANTIGEN AND THE PROLIFERATING ACTIVITY IN HEPATOCELLULAR CARCINOMA / 解放军医学杂志

ABSTRACT

To investigate the expression of cytokeratin 18(CYK18) in hepatocellular carcinoma(HCC) cell membrane and the relationship between the serum levels of tissue polypeptide specific antigen (TPS) and the proliferating activity of HCC cells. The serum levels of TPS were determined by TPS TM ELISA in 96 patients with HCC. Four micrometer paraffin embeded sections of liver specimens from these patients were immunostained with the strept avidin biotin complex immunoperoxidase technique (IHC SABC). All of the HCC preparations were positively stained by CYK 18 and proliferating cell nuclear antigen (PCNA) with IHC SABC.The results of PCNA stainings were + in 62 5%, in 26 0%,  in 8 3%,and  in 3 1% .The median levels of TPS in the four groups were + 137 98 U/L,  685 3 U/L,  in 1 126 U/L and  4 672U/L respectively. There were significant differences among these groups ( P