Effects of cardiac ?_1-adrenergic receptor on myocardial remodeling and modulating calcium channel / 中国实用内科杂志

ABSTRACT

Objective To inquire into the effects of cardiac ? 1 -adrenergic receptor on myocardial remodeling and modulating calcium channel. Methods The models of myocardial remodeling after myocardial infarction in Wistar rats were used to study the expression changes of cardiac collagen subtype Ⅰ,Ⅲ,fibronectin (FN) with immunohistochemistry,and changes of T-type calcium channel,L-type calcium channel and ? 1 -adrenergic receptor mRNA expressions by reverse transcription and polymerase chain reaction(RT-PCR) and in situ hybridization. Cardiac total Ca 2+ was tested by atomic absorption/flame emission spectrophotometer. Results Cardiac collagenⅠ,collagen Ⅲ,FN protein expressions in betaloc group were remarkably decreased (P