The Regulators of VEGF Expression in Mouse Ovaries
Yonsei Medical Journal
;
: 679-686, 2005.
Artículo
en Inglés
| WPRIM
| ID: wpr-55371
ABSTRACT
The objectives of this study were to explore whether ovarian vascular endothelial growth factor (VEGF) expression in mice can be regulated by IL-6 (interleukin-6), angiotensin II, FSH, and hCG; and to test whether the mouse ovarian VEGF expression can result in angiogenesis. The ICR mice were sacrificed, and their ovaries were recovered. Recovered ovaries were treated with IL-6, angiotensin II, FSH, and hCG separately and incubated for 24 hours in alpha-MEM. Expression of mRNA and protein of VEGF were assessed by RT-PCR and immunohistochemistry. The resulting angiogenesis was evaluated through immunohistochemical analysis for CD34. Treatment of mice ovaries with IL-6, FSH, and hCG resulted in a significant increase of VEGF mRNA, and IL-6 was the most potent inducer of VEGF. IL-6 and FSH resulted in increased neovascularization in the follicular phase of mouse ovaries. In contrast, angiotensin II could not increase VEGF expression or neovascularization. We documented an in vitro increase in VEGF expression by IL-6, FSH, and hCG; and reaffirmed that the proliferative response of murine ovarian endothelial cells paralleled an increase of VEGF expression.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ovario
/
ARN Mensajero
/
Inmunohistoquímica
/
Regulación de la Expresión Génica
/
Interleucina-6
/
Antígenos CD34
/
Factor A de Crecimiento Endotelial Vascular
/
Hormona Folículo Estimulante
/
Gonadotropina Coriónica
/
Ratones Endogámicos ICR
Límite:
Animales
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2005
Tipo del documento:
Artículo
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