CLONING OF THE HEPATITIS C VIRUS NONSTRUCTURAL PROTEIN 5A BINDING PROTEIN 37 CODING GENE / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)2001.
Artículo
en Chino
| WPRIM
| ID: wpr-554240
ABSTRACT
The nonstructural protein 5A (NS5A) of the hepatitis C virus (HCV) has been shown to interact with a variety of cellular proteins and implicated in the regulation of cell growth, interferon resistance, and other cellular signaling pathways. Using the yeast-two hybrid method, we have isolated a clone that encodes a novel NS5A--associated binding protein NS5ABP37. Reverse transcription polymerase chain reaction (RT-PCR) method was employed to amplify the full fragment,and the plasmid pGADT7-NS5ABP37 with the Saccharomyces cerevisiae vector pGADT7 was constructed. To prove the interaction, yeast cell Y187 transformed with pGADT7-NS5ABP37 was mated with yeast cell AH109 containing pGBKT7-NS5A to verify the interaction between the novel protein coded by the new gene NS5ABP37 and NS5A.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Medical Journal of Chinese People's Liberation Army
Año:
2001
Tipo del documento:
Artículo
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