FURTHER OBSERVATION ON DYNAMICS OF IN VITRO GRANULOMATOUS RESPONSES SURROUNDING SCHISTOSOME EGGS / 中国血吸虫病防治杂志

ABSTRACT

Objective To further observe the morphologic changes of in vitro model of granulomatous responses around Schistosoma japonica eggs and determine the relationship of reaction inten-suy to infection stage of those schistosome-infected mice from which the splenocytes were obtained for in vitro experiments.Methods The spleen cells isolated from schistosome-infected mice were incubated and dry schistosome eggs were added into those cultures. These splenocytes responses surrounding eggs were observed and counted under the microscope.Results The splenocytes from infected mice showed obvious adherent responses to the dry eggs. Adhering cells were,at first,granulocytes,then replaced by the macrophages. Around the eggs proliferating lymphocytes,later fibroblasts, were found. The reaction index (RI) of the spleen cells from infected mice were significantly higher (P