In vitro culture of human Endometrial cells / 医学研究生学报

ABSTRACT

Objective:To establish a method of endometrial glandular cells culture,storage and subculture in vitro,so as to study its antigen components.Methods: Endometrial glandular cells and stromal cells were isolated by collagenase digestion and copper sieve filtration methods and monolayer cultured. Results: Glandular cells and stromal cells in the culture had been observed by light microscopy and stained positively by keratin and vimentin McAb respectively using immunohistochemical method. The glandular cells life span can last 4-6 weeks and 2-3 population doublings. Conclusion: Our establishment of endometrial glandular cells show great promise for providing more non-hysterectomy origin EMAg.