Role of NF-kappa B in pathogenesis of acute liver injury in burn rat with sepsis / 解放军医学杂志

ABSTRACT

Objective To investigate the potential role of nuclear factor kappa B (NF-?B) in the development of acute liver injury induced by postburn sepsis in rats. Methods Sixty-three Wistar rats were randomly divided into normal control group, burn sepsis group, burn sepsis with pyrrolidine dithiocarbamate (PDTC) treatment group, and simple PDTC treatment group. Rats in experimental groups were subjected to 30% full-thickness scald injury combined with intraperitoneal injection of lipopolysaccharide (LPS) to mimic early sepsis after burns. The following parameters were measured with the method indicated in parentheses NF-?B activity (electrophoretic mobility shift assay, EMSA), I?B-? expression (Western blotting), TNF-? (enzyme-linked immunoadsordent assay, ELISA). Results NF-?B activity was markedly enhanced and reached its peak at 1 hour after LPS challenge (P