Effects of aging on testicular steroidogenic function in rats / 第二军医大学学报

ABSTRACT

Objective:To study the changes of testicular steroidogenic acute regulatory protein (StAR), 17?-hydroxys-teroid dehydrogenase (17?-HSD) and aromatase (P450arom) in elder SD rats, exploring the effects of aging on testicular steroidogenic function. Methods:The young male rats and old male rats were treated with human chorionic gonadotropin (hCG) for 3 times and then rat serum testosterone(T) and estradiol(E2) levels were determined by chemiluminescentenzyme immunoassay(CLIA) and the mRNA levels of StAR, 17?-HSD Ⅲ and P450arom were determined by RT-PCR. Results: (1) The levels of serum T and 17?-HSD Ⅲ mRNA of old SD rats were significantly lower than those of young rats before and after hCG stimulation (P