The effect of ClC-3 antisense oligonucleotide on apoptosis induced by thapsigargin in PC12 cells / 中国药理学通报

ABSTRACT

AIM To investigate the effect of ClC-3 antisense oligonucleotide on apoptosis induced by thapsigargin in PC12 cells. METHODS Western-blot was performed to detect the protein expression of ClC-3 in PC12 cells. MTT assay was used to measure the effect of ClC-3 antisense oligonucleotide on growth inhibition induced by thapsigargin. The effect of ClC-3 antisense oligonucleotide on apoptosis was studied with the fluorescent microscopy, DNA agarose gel electrophoresis, flow cytometry analysis. RESULTS Compared with control group, transient transfection of PC12 cells with antisense oligonucleotide specific to ClC-3 caused an inhibitory effect on expression of ClC-3 protein in a time-and concentration-dependent manner,whereas the thapsigargin-induced reductions of viability of PC12 cells and apoptosis were markedly enhanced (P