Epigallocatechin-3-gallate Inhibits the Expression of Adhesion Molecules by Blocking Nuclear Factor Kappa B Signaling in Intestinal Epithelial Cells
Intestinal Research
;
: 261-267, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-55530
ABSTRACT
BACKGROUND/AIMS:
Epigallocatechin-3-gallate (EGCG) is the main polyphenol in green tea and has anti-inflammatory and anti-oxidative effects. The aim of this study was to determine the impact of EGCG on the expression of adhesion molecules and lipopolysaccharide (LPS)-induced nuclear factor-kappa B (NF-kappaB) signaling in rat intestinal epithelial (RIE) cells.METHODS:
The effect of EGCG on LPS-induced NF-kappaB signaling and expression of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 was examined by reverse transcription polymerase chain reaction, western blotting, immunofluorescence and electrophoretic mobility shift assay.RESULTS:
LPS-induced expression of ICAM-1 and VCAM-1 mRNA was inhibited by EGCG treatment in RIE cells. LPS-induced inhibitor of kappa B alpha degradation and NF-kappaB nuclear translocation were blocked by EGCG in RIE cells. EGCG blocked LPS-induced NF-kappaB DNA-binding activity in RIE cells. The pharmacological NF-kappaB inhibitor Bay11-7082 suppressed the LPS-induced expression of ICAM-1 and VCAM-1 mRNA in RIE cells.CONCLUSIONS:
These results indicate that EGCG inhibits LPS-induced ICAM-1 and VCAM-1 expression by blocking NF-kappaB signaling in intestinal epithelial cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sulfonas
/
Té
/
ARN Mensajero
/
Catequina
/
Western Blotting
/
Reacción en Cadena de la Polimerasa
/
FN-kappa B
/
Técnica del Anticuerpo Fluorescente
/
Molécula 1 de Adhesión Intercelular
/
Molécula 1 de Adhesión Celular Vascular
Límite:
Animales
Idioma:
Inglés
Revista:
Intestinal Research
Año:
2013
Tipo del documento:
Artículo
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