Interleukin-2? receptor in membrane lipid rafts / 肠外与肠内营养
Parenteral & Enteral Nutrition
;
(6)2004.
Artículo
en Chino
| WPRIM
| ID: wpr-555571
ABSTRACT
Objective:
To determine lipid rafts on the membrane of T cells is the functional microdomains for IL-2? receptor.Methods:
Flow cytometric analysis was performed for expression of CD25(IL-2R?) on the surface of T cells. Lipid rafts were isolated by discontinuous sucrose density gradient ultracentrifugation. The localization of IL-2R? in fractions isolated from sucrose density gradients was determined by immunoblotting and detected by chemiluminescence.Results:
cells were stimulated with IL-2 and expression of CD25(IL-2R?) on the surface of T cells was 37.08%. Immunoblot analysis of fractions from sucrose gradients revealed that a large proportion of IL-2R? was localized in lipid rafts.Conclusions:
lipid rafts is the functional microdomains for IL-2? receptors.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Parenteral & Enteral Nutrition
Año:
2004
Tipo del documento:
Artículo
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