Reproduction of experimental saccular aneurysm by corrosion of arterial wall with elastase / 解放军医学杂志

ABSTRACT

Objecive To reproduce an experimental model of aneurysm more which is simulating intracranial aneurysm pathomorphologically to serve further research on aneurysm. Methods Saccular aneurysms of the carotid artery were reproduced by corrosion of initial segment of the external carotid artery (1mm long) with elastase in 10 SD rats. After 4 weeks, the size of the aneurysms was measured. Then the aneurysms were fixed by intra-arterial perfusion of formalin and were resected. The pathological characteristics were evaluated with hematoxylin-eosin and Van Gieson staining. Results The pathological features of the experimental aneurysms showed that the tunica intima was interrupted, the elastic and muscular layers were disrupted or disappeared, replaced by proliferative collagen fiber, simulating natural aneurysms. After 4 weeks, the size of aneurysms was larger compared with that in the beginning (P