Effect of Cyclosporin A and Tetrandrine on rhodamine 123 accumulationin in bovine brain capillary endothelial cells / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12)2003.
Artículo
en Chino
| WPRIM
| ID: wpr-555725
ABSTRACT
Aim To study the effect of cyclosporin A and tetrand ri ne on P-glycoprotein (P-gp)of bovine brain capillary endothelial cell. M ethods The fluorescent dye, rhodamine-123 (Rh-123) was used to evaluate t he functional activity of the P-glycoprotein (P-gp) efflux transport system in primary cultured bovine brain capillary endothelial cell (BCEC) monolayer. Results Rhodamine-123 accumulation was increased significantly in monola yer treated with the P-gp modifying agent, cyclosporin A and tetrandrine. Conclusion The observation suggests that this Rh-123 method is sens itive, stable to evaluate the function of P-gp of blood-brain barrier (BBB). R h-123 accumulation is also increased by tetrandrine in dose-dependent manner.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Pharmacological Bulletin
Año:
2003
Tipo del documento:
Artículo
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