Isolation purification and characterization of NV-PP-1 from Nidus vespae / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 233-1999.
Artículo
en Chino
| WPRIM
| ID: wpr-555797
ABSTRACT
OBJECTIVES:
To isolate and purify a new peptide named as NV-PP-1,from anti-inflammatory protein extracts of Nidus Vespae and to characterize it.METHODS:
It was purified by ammonium sulfate precipitation,DEAE-Sephadex A50 ionexchange chromatography,Sephadex G-50 gel filtration and HPLC,respectively.Then it was characterized by HPLC on Protein-PakTM-60 column,IEF-HPCE(isoelectric focusing-high performance capillary electrophoresis),UV spectrum,amino acid analysis,and the N-terminal analysis with DNS-Cl.RESULT:
Both HPLC on Protein-PakTM-60 column and IEF-HPCE showed its homogeneity.Its molecular weight was estimated to be 7.079 kD by HPSEC.UV spectrum indicated its maximum absorption at 274nm.Its PI was determined to be 4.08 according to IEF-HPCE.The terminal analysis showed its N-terminal amino acid was blocked.Amino acid analysis of NV-PP-1 indicated that it was rich in Ser,Glu,Gly.CONCLUSION:
NV-PP-1 is the first peptide isolated from the anti-inflammatory protein extracts of Nidus vespae.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Pharmaceutical Journal
Año:
1999
Tipo del documento:
Artículo
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