Experimental study on the inhibitory effect of IL-4 on IL-1?-induced mesangial cells proliferation in rat / 解放军医学杂志

ABSTRACT

Objective To observe the inhibitory effect of IL-4 on the IL-1?-induced rat mesangial cell proliferation, the release of PGE 2, and on the COX-2 mRNA and protein expression, so as to explore the related mechanism of anti-glomerulosclerosis. Methods Rat mesangial cell (MC) was cultured in vitro and the IL-1? -induced proliferation of MC was detected by MTT method. ELISA method was employed to measure the contents of PGE 2 in blank control group, IL-1? group, IL-4+IL-1? group, NS-398 group, NS-398+IL-1? group, indomethacin group, indomethacin+IL-1? group. The expression of mRNA and protein of COX-1 and COX-2 gene were detected by RT-PCR and Western blot, respectively. Results IL-1? could induce the proliferation of MC when the concentration was 0.1-100ng/ml in 12h, 24h and 48h. When its concentration was 2ng/ml, it had the strongest effect in 24h. All the IL-4, NS-398, indomethacin could inhibit IL-1? to induce MC producing PGE 2. The inhibitory rates are in proper order as IL-4 (84.9%), NS-398(56.5%), and indomethacin(41.2%). Both IL-4 and NS-398 could obviously inhibit the expression of COX-2 mRNA and protein proved by RT-PCR and Western blot. Indomethacin could strongly inhibit the expression of COX-1 mRNA and protein, while only shew a weak inhibition on the expression of COX-2 mRNA and protein. Conclusion IL-4 could markedly inhibit the IL-1? -induced MC proliferation, down-regulate COX-2 gene, decrease the production of PGE 2, and thus take effect on anti-glomerulosclerosis.