Interleukin-10 promoter single nucleotide polymorphism and acute rejection in renal transplantation: a donor-recipient paired study / 第二军医大学学报

ABSTRACT

Objective:To study the influence of 3 single nucleotide polymorphisms (SNP) of IL-10 promoter (-1 082, -819 and -594) on acute rejection after renal transplantation in a donor-recipient paired mode. Methods: Three IL-10 promoter SNPs of 52 donor-recipient pairs were genotyped and phenotyped by PCR using sequence-specific primers (SSP). Recipients were followed up for 1 year after transplantation, and acute rejection episodes were observed. Incidences of rejection were compared against pair patterns and differences were analyzed using Fisher's exact test. Results: No genotype of high producer was found in any donor and recipient in this study. Forty-nine recipients were followed up for over 12 months. Of them, 18 recipients (36.7%) had acute rejection and 7 recipients (14.3%) had multiple rejection(MR). The relationship between donors with intermediate producer and acute rejection and MR tended to be statistically significant (P=0.076, P=0.051).The MR rate increased significantly in the pair of donor intermediate producer/recipient low producer (P=0.036). Conclusion: It is preferable to take donor’s IL-10 SNP into consideration in study of IL-10 SNP and renal transplantation. Donor intermediate producer can increase the risk of acute rejection. The combination of donor intermediate/recipient low producer can increase the risk of MR.