Neuroprotective effect of heme oxygenase-1 induced by hemin in the brain of rats suffered from ischemia-reperfusion injury / 第三军医大学学报

ABSTRACT

Objective To investigate the neuroprotective effect of heme oxygenase-1 induced by hemin on the rat brain after ischemia-reperfusion. Methods The global cerebral ischemia-reperfusion model in 48 Wistar rats was surgically prepared by four-vessel occlusion method (4-VO) and another 8 rats underwent sham-operation as normal control. Hemin was injected over 5 min at the lateral ventricle at 15 min, 24 h and 48 h after reperfusion in 24 ischemia-reperfusion rat models and normal saline was applied to another 24 rat models at the same time points as negative control. The effect of hemin was evaluated on the pathologic outcome and on the immuohistochemical reaction for HO-1 after 10-minute global cerebral ischemia followed by 24 h, 48 h and 72 h reperfusion. Results The peak level of HO-1-like immunoreactivity was found at 48 h after the reperfusion as compared with the matched rats in normal saline group (P