Study on the antiviral effect of C gene truncated HBV mutant in HBV infection / 中华传染病杂志
Chinese Journal of Infectious Diseases
; (12)2001.
Article
en Zh
| WPRIM
| ID: wpr-557423
Biblioteca responsable:
WPRO
ABSTRACT
Objective To explore the effect of the C gene truncated HBV mutant on HBV replication. Methods The C gene truncated HBV vector pHBV-?C was constructed through the molecular clone in vitro,and then transfected transiently into HepG2 cell.The expression of S protein was assayed by ELISA and SDS-PAGE Western blot.After co-transfection with pHBV-?C and wild HBV genome adwR9 into HepG2 cell the DNA was detected quantitatively by real-tine fluorescence quantitative PCR in the culture medium and the cell. Results There was no significant difference in expression of S protein assay by ELISA and Western blot.The DNA of the cotransfected group with pHBV-?C and adwR9 was lower than that of control group in the culture medium and the cell. Conclusions(C gene) truncated HBV mutant can cause the reduction of HBV replication.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Infectious Diseases
Año:
2001
Tipo del documento:
Article