A high-throughput fluorescent polarization model for screening EPO receptor ligand / 中国药理学通报

ABSTRACT

Aim To discover lead compound whose function is similar to EPO.Methods A high-throughput Fluorescent Polarization(FP) assay was established for screening ligand of EPO receptor from small molescules and natural products.In the FP assay,EPO receptor was abstracted from FVA cell.EPO was labeled with FITC.Results The optimized concentration of FITC-EPO was 10~(-9) mol?L~(-1).Different concentrations of DMSO(VDMSO/Vtotal 0%,0.5%,1%,2.5%,5%) didn′t affect the FP assay.The value of Z′ in the FP assay was 0.78.Conclusion The assay was robust and of high sensitivity.