A simple way to improve fibroblast seeding efficiency and uniformity in constructing 3-D dermal equivalent / 第二军医大学学报

ABSTRACT

Objective:To explore a simple way to improve seeding efficiency and uniformity in constructing 3-D dermal equivalent,in order to effectively construct tissue engineered skin.Methods: Human dermal fibroblasts were added onto collagen sponge scaffolds and cultured in the following 3 ways(1)oscillating seeding and oscillating culture(OO),(2)oscillating seeding and static culture(OS),and(3)static seeding and static culture (SS).Samples were obtained and subjected to light microscopy,scanning electron microscopy(SEM) and quantitative cell number assay at planed times.Results: Oscillating seeding achieved more uniform spatial cell distribution within scaffolds as compared to static seeding.Moreover,fibroblast seeding efficiency was significantly higher in oscillating conditions(\%) than in static conditions(\%,P