Pathology of an Aplastic Anemia Model Induced by Benzene in Mice / 医学研究杂志

ABSTRACT

Objective To develop a model of be nzene induced aplastic anemia in mice and to observe pathology of aplastic anemi a induced by benzene;Methods we tried to establish a model of benzene induced aplastic anemia in mice,then we studied pathology of aplastic anemia and the pr evention of amifostine on the model.(1) CD1 male mice were injected subcutaneou sly with benzene +corn oil three times a week,and randomly assigned to four gro ups according to the dose of benzene administrated:B1group(0.5ml/kg),B2group( 1.0ml/kg),B3group(1.5ml/kg),B4 group(2.0ml/kg),C group (injected intraperi toneally with 200mg/kg amifostine 30min before benzene administrated on the basi s of the aplastic anemia model as amifostine treated group),while A group treat ed with only corn oil as control,all the groups were complement with corn oil t o 4ml/kg;Before and 2 days after benzene were administrated 10,15,20 and 25 t imes respectively,all the groups mice were killed respectively ,the parameters tested were as following:general condition,peripheral blood smear,histopatho logy of bone marrow 、liver,spleen and PCNA,TUNEL.Results Compared with the control group,some parameters were changed in each model group,but only the pa rameters in B4 group were significantly different from the control group.After 25 times of benzene administration,compared with the control group (A group),B4 group mice showed 18.51% body and 63.86% spleen weight loss (P