A preliminary study of single nucleotide polymorphisms of thrombospondin-1 gene in acute coronary syndrome disease / 中国实用内科杂志

ABSTRACT

Objective This study investigated into the relationship between single nucleotide polymorphisms(SNPs)of thrombospondin-1 gene(TSP-1)and acute coronary syndrome(ACS).Methods From Nov.2003 to Sep.2005,fragment of exon thirteen in TSP-1 gene from 323 cases of ACS and 273 subjects without coronary heart disease were analysed by polymerase chain reaction and restriction fragment length polymorphism,and sequence analysis was done for confirmation in the First Affiliated Hospital of Nanjing Medical University.Results Of the 596 subjects participating in the study,only 6 of the heterozygotes and none of the homozygotes were detected for the 700S allele.The prevalence of the 8831G in the healthy controls was less frequent than those of the west populations(0.4%vs 11%,P