Measurement of recombinant human glucagon-like peptide-1(7-36) concentration by ELISA kit methods / 中国临床药理学与治疗学

ABSTRACT

AIM: To qualitatively detect the concentration of recombinant human glucagon-like peptide-1(7-36)[rhGLP-1(7-36)] in human blood.METHODS: RhGLP-1(7-36) concentrations of standard and blood samples were detected by ELISA kits.RESULTS: The parameters of specificity,sensitivity,detection limits,reproducibiltiy and recovery can meet the requirements for pharmacokinetic studies.CONCLUSION: The concentration measuring method of rhGLP-1(7-36) in blood samples using ELISA kit produced by LINCO company can meet the detection methods of biological production,which can be used as a measuring method of rhGLP-1(7-36) concentration and for its pharmacokinetic studies.