Inhibitive effect and mechanism of PPAR? on the ECM production of mesangial cells induced by angiotensin Ⅱ / 中华肾脏病杂志

ABSTRACT

Objective To study the inhibitive effect and mechanism of PPAR?1 on the extracellular matrix (ECM) accumulation of mesangial cells induced by Ang Ⅱ .Methods The plasmid of PPAR?1/WT (wild type) was transfected into mesangial cells. After 48 hours of Ang Ⅱ stimulation, the gene expression of TGF-?1, PAI-1, c-fos and c-jun was examined by RT-PCR. Protein levels of p-ERK, I-?B and nucleus/cytosol ratio of NF-?B were estimated by Westen-blot. The concentrations of FN and TGF-?1 were estimated by ELISA. The activity of PPAR?1 was examined by specific PPRE binding activity. Plasmid expressing non-functional dominant negative type of mPPAR?1, pIRES2-EGFP-mPPAR?1/DN (DN), and blank plasmid, pIRES2-EGFP (Blank) were used as controls. Effects of 6 ?mol/L PPAR? agonist pioglitazone (Pio) were also studied. Results The expression of TGF-?1 and PAI-1 mRNA in mesangial cells induced by Ang Ⅱ was inhibited by PPAR?1(P