Characterization of target antigen of new monoclonal antibody 03.2C1-C2 specific for Candida albicans germ tube / 第三军医大学学报

ABSTRACT

Objective To analyze the characterization of the target antigen of the monoclonal antibody(Mab) 03.2C1-C2 against germ tube of Candida albicans,and explore the possibility of Mab03.2C1-C2 application in the laboratory examination.Methods SDS-PAGE and Western blotting were used to analyse the molecular weight of antigens recognized by Mab03.2C1-C2.The distribution of the target antigens on the surface of the germ tube induced in vitro were analyzed.The epitope of the target antigen were analysed via indirect immunofluorescence(IIF).Results The target antigen recognized by Mab03.2C1-C2 could be detected 30 min after the germ tube formation.The distribution of the target antigens were different between the germ tube formated in vitro and in vivo.The epitope might locate in the N-carbohydrate chain.Conclusion New protein-glycoprotein that is the target antigen of Mab03.2C1-C2 was produced during the formation of the C.albicans germ tube.Strong antigenicity of glycoprotein is of certain value on quick diagnosis of Systemic C.albicans.