Effect of paeonol on proliferation of vascular smooth muscle cell in atherosclerosis rabbits / 中国药理学通报

ABSTRACT

Aim To study the effect of paeonol on proliferation of vascular smooth muscle cell in atherosclerosis rabbits.Methods Atherosclerosis model on rabbits was established by high fat diet.The pathologic changes of aortic blood vessel were observed by lightmicroscope.The effect of paeonol on the proliferation of vascular smooth muscle cell in vitro was analyzed by MTT method.The lipid,SOD and MDA contents in aorta tissue were assayed by employing the automatic biochemistry analyzer and enzymic method respectively.IL-1?,TNF-? were quantified by radio immunoassay.The expresstion of PCNA protein was assayed by immunohistochemical method.Results Treated with Paeonol(75,150 mg?kg~(-1)) by interagastric administration for 6 weeks,the thickness of vascular tunica intima was observed and the number of foam cells was decreased in atherosclerosis rabbits.Paeonol(100～300 mg?L~(-1)) could inhibit the proliferation of cultured vascular smooth muscle cell and diminish the level of TC,MDA,pro-inflammatory cytokines,as well as enhanc the concentration of SOD in aortic tissue of atherosclerosis rabbits.The expressing intensity of PCNA protein was weakened.Conclusion Paeonol could inhibit the proliferation of vascular smooth muscle cell in vivo and in vitro,which may be related to decrease of contents of lipid,the extent of lipid peroxidation and the concentrations of proinflammatory cytokines.Paeonol might regulate the proliferation periods of vascular smooth muscle cell through inhibiting the expression of PCNA protein.