ROS level and ASK1 protein expression in myocardium during chronic pressure overload in rat left ventricle / 第三军医大学学报

ABSTRACT

Objective To study the effect of apoptosis signal regulating kinase 1(ASK1) protein expression in hypertrophic myocardium in rat models of hypertension.Methods The rat model of hypertension was established by abdominal aortic constriction.Six weeks later,the reactive oxygen species(ROS) level and ASK1 protein expression in myocardium of left ventricle were detected respectively by color matching and Western blotting.Results The left ventricular mass index,ROS level and ASK1 protein expression all increased significantly in hypertensive rats as compared with those underwent sham operation.Conclusion Under chronic pressure overload,the activated ASK1 with the increasing ROS may play an important role in signal transduction pathway of myocardial hypertrophy.