Study of peptides antigen of the dendritic cell from human cord blood on specific cytotoxic T iymphocytes / 中国基层医药

ABSTRACT

Using pH 3.3 Citrate-phosphate buffer(0.13)mcitric acid/0.066 M Na_2 HPO_4)elution tech- nique,peptides antigen were extracted on the surfacd of QGY-7703 cell.With the peptides antigen(50?g/ml)den- dritic cells were processing ang presenting.The dendritic cells were from human cord blood stem cells induced by granulocyte-macrophage colony stimulating factor(100ng/ml).Tumor necrosis factor(1000U/ml)and interlukin-4 (50ng/ml).The processed peptides antigen were able to activate the peripheral blood T iymphocytes and changed them into specific cytotoxic T iymphocytes could recognize the QGY-7703 antigen peptides by the T cell receptor and then killed the target cell-QGY-7703 cell.The test were shownat effective celltarget cell(ET)over 501,a- long with growth of ET ratio,target cell special lysis percentage obviously growing.The most cytotoxicity(%) could reach 61.65 at the condition of ET=2001.In the study we also have known that the test to QGY-7703, SPC-A-1,HL60 as well as K562 cell lysis were indicated this killed activation is of specialty.The cause of target cell death is induced by programmed call death(PCD)or apoptosis way.In the processing of the test using dendritic cell dealing with special tumor peptides antigen the total cytotoxcity growed highest,reaching 34.14.The result furtherly demonstrated,dendritic cell originating fron CD_(34)~+ umbilical hemopoietic stem cell could process ang present peptides antigen effectively as antigen present cell(APC)and activated the cytotoxic T iymphocytes(CTL).