Study on the effect of nerve growth factor on Th1/Th2 cell immune imbalance in asthmatic rats / 中国实用内科杂志

ABSTRACT

Objective To study the effect of nerve growth factor on airway inflammation and Th1/Th2 cells immune imbalance.Methods(1)32 SD rats were divided into 4 groups randomlyasthma,control,NGF,anti-NGF-antibody groups.The asthmatic model were established by inhalation and injection of ovalbumin.(2)We investigated the expression of NGF mRNA in the lungs in asthma and control groups by reverse transcription-polymerase chain reaction(RT-PCR).(3)In the lungs of the four groups,the expression of interferon-?(IFN-?,one of the Th1 cytokines),interleukin-4(IL-4,one of the Th2 cytokines)mRNA was detected by RT-PCR too.Results(1)As compared with the control group,NGF mRNA was significantly enhanced in the asthma group[(90.4?7.6)% vs(51.8?12.3)%,P